This study aimed to explore the tones of Miaoli Sixian Hakka (MSH) under two methods: traditional survey and acoustic analysis. This study reviewed the existing literature of MSH citation tones under the two methods, and stated notational similarities and differences of the citation tones, especially the Yangping and Yinru tones. Then, this study compared three tonal normalization formulas by analyzing lexical tones in MSH. Twenty native speakers of MSH joined this study. The speakers were asked to read a word list in which each lexical tone had three word tokens, and a total number of 54 sound tokens were created by each informant. Their speeches were recorded for acoustic analysis. PRAAT was used to averagely seprate F0 contours into 11 points. Values of fundamental frequency (F0) of every point of each lexical tone were measured. All fetched F0 values were then normalized by T-value, LZ-value, and semitone-value (ST) formulas. Results showed that the normalized tonal values of the semitone-value formula were more similar to those of traditional survey. However, great difference in tonal values also existed in Yangping and Yinru between the two methods in terms of all formulas. This study also showed tonal systems of six Hakka dialects, and found systematically notational differences in low-level tones between the two methods (i.e., [11] vs. [31]). Finally, this study provided explicable accounts for the tonal notational asymmetry in terms of physiological restrictions, the difference between phonetics and phonology, the distinction between speech production and perception, tonal contrast and exclusion, and the phonetic features of level tones originated from Middle Chinese.