The purpose of this study was to investigate the interrelationship among frustration experience, frustration endurance and failure response of the athletic delegations in National Cheng-kung University (N.C.K.U.). In addition, further regression prediction analysis and comparison among frustration experience, frustration endurance and failure response of students with different background were also performed. Male (n = 117) and female (n=84) subjects of present study were from athletic delegations of N.C.K.U. Subjects were tested with questionnaire designed by Lee for investigating their interrelationship among frustration experience, frustration endurance and failure response. Results were analyzed by using factor analysis, one-way ANOVA, Scheffe's posterior comparisons and stepwise regression. Conclusion: 1. Comparisons of frustration experience, frustration endurance and failure response between different gender showed that interpersonal frustration experience was significantly higher in male subjects then in female subjects. In addition, behavior response was significantly higher in female subjects. 2. Tests in frustration experience, frustration endurance and failure response of subject with different exercise capability showed that national level athletes were significantly higher in study behavior then college level athletes. And, national level athletes were significantly higher in total frustration endurance then college level athletes. 3. The regression formulae of background factor, frustration experience, frustration endurance for failure response were listed below: A. Recognition response = 8.75 + 0.188 × total study frustration endurance - 0.33 × exercise experience + 0.12 × exercise action orientation + 0.10 × interpersonal behavioral orientation B. Behavioral response = 4.44 + 0.39 × exercise emotion perception + 0.37 × study behavioral orientation + 0.24 × exercise venture + 0.08 × interpersonal frustration experience C. Sentimental response = 9.18 + 0.14 × exercise frustration experience + 0.26 × studying emotional perception - 0.38 × exercise experience + 0.56 × interpersonal emotional perception + 0.18 × exercise action orientation -0.39 × total interpersonal frustration endurance D. Total failure response = 21.57 + 0.43 × total studying frustration endurance + 0.29 × total exercise frustration endurance - 0.73 x exercise experience - 0.62 × interpersonal venture + 0.41 × interpersonal emotion perception 4. Non-contacted exercise athletes were higher in studying action orientation then contacted exercise athletes. In addition, contacted exercise athletes were significantly higher in exercise venture then non-contacted exercise athletes. In emotional response, non-contacted exercise athletes showed significantly higher value then contacted athletes did.