Society is entering into a global an diverse century. Students can hardly be expected to adapt in their future work and lives if they are taught yesterday’s knowledge and skills at school. Hence the promotion of Information and Communication Technology (ICT) and the handing of information in education is an important target for education reform in the modern countries of the world. Not only should teachers and students have basic ICT knowledge and schools provide an accessible ICT teaching-learning environment, but a good quality course also plays a key role through the use of ICT in education. The use of ICT can make course so fascinated that will raise students’ interest in learning and improve their learning achievement. The purpose of this study was to develop a computer-assisted instructional software for biology education, and to assess it’s effectiveness on students learning when combining with different instructional strategies. The process of developing the software started from collecting and analysing teaching materials. Falsh5 was then used to design the content and animation. The course is divided into four units, i.e. heart, blood vessels, blood and circulation. It is also combined with online assessment to evaluate students’ learning achievement. In order to examine the feedback from both teachers and students about the effectiveness of using the designed AI as a teaching and learning tool, two instructional methods were conducted in the classroom. One was the use of a computer note book connected to a projector to present the content of the CAI. The other was the provision of a network to students, so that they could learn by means of the Internet by themselves with the help of teachers. During the process of learning, students could post their questions on the discussion board and discuss them with each other. At the same time, students could also improve their abilities to express, communicate and share information with others. The findings of the research reveal that teaching biology through ICT captured student’s interest and their attention, which in turn had a positive impact on their achievements. The results also suggest that integrating ICT and two instructional methods can help teacher save instructional time and facilitate students’ understanding, even some low achievement students were deeply impressed by the content of the animation and benefited from it. The whole process of the research which included designing course combined with CAI, establishing an ICT environment in schools and teaching was well recorded and discussed. It is believed that this data can contribute to the promotion of ICT in the teaching of biology. ICT and the World Wide Web (WWW) are changing people’s learning styles. As educators, it is our goal to support the implementation of the nine-year compulsory education by using ICT and the WWW to create an effective, autonomous and innovative learning environment for students and promote their lifelong learning.