The purposes of this research are to discuss the reactions of people’s emotional experience and landscape preference on the visual effects of street signs, the relationship between emotional experience and landscape preference, and the impact of street signs and emotional experience on landscape preference. There are 80 streetscape pictures based on 20 unified sign streets. Independent variables such as sizes, styles, positions and materials of street sign, along with 4 emotional factors are selected to understand the relationship with streetscape preference. The result of this research refers the followings. The three most preferred streets are Da-lung Road in Taichung City, Sec. 3 of Lin-kang Road in Wuchi Town, and the old Hoping Street in Da-hsih Town. Other research results find that 1.Size, position, and material of street sign had significant influences on people’s emotional experiences. 2.Only size and position of street sign had impacts on observers’ preferences. 3.Positive pleasure, negative e pleasure and low arousal factors worked together to play a significant role on influencing preference. 4.Combined both physical and psychological factors. Only positive pleasure and negative pleasure had significant influences on people’s landscape preference.