The main objective of Health-related Fitness in Chia-Nan University was to increase students' physical fitness and reducing exercise injury and accident of sudden death. Chia-Nan University authority insisted of using 3 minutes step-test instead of 800/1600M test. Therefore, Chia-Nan University established students' physical fitness passport for student to record the value of physical fitness measurement. The main purpose of this study was : 1. to understand Health-related fitness of Chia-Nan University students, and to establish a percentile rank scale; 2. to understand the difference between male and female concerning Health-related fitness. The results showed that (N=404, Male=120, Female=284): 1. The mean B. M. I. of male and female subjects was 22.51 and 21.56 respectively; 2. The mean Flexibility of male and female subjects was 32.10 cm and 33.91 cm respectively; 3. The mean Muscular Fitness of male and female subject was 38.75 times and 28.84 times respectively; 4. The mean Explosive Strength of male and female subjects was 206.74cm and 141.16cm respectively; 5. The mean Physical Index of male and female is 50.55 and 49.84. There is apparent difference between male and female concerning Muscular Fitness and Explosive Strength.