The purpose of this study was to investigate the impact of variation of run-up velocity curve and utilization rate of run-up speed on long jump. Twenty males studying in the Department of Physical Education served as participants, and their mean age, height and weight were 18.90±1.12years, 175.30±5.07 cm and 65.60±7.42 kg respectively. The Laster Distance Measurement 300 C system (100Hz) was used to record and analyze the full process of 50 meters and long jump kinematics data. Repeated measures t-test and the Pearson’s product-moment correlation were conducted. The summary of results was listed as follows： The participants reached the fastest velocity at the section of 30-35 meters during 50-meter sprint. The performances of long jump by 13-step and 17-step run-up were better than that by 9-step run-up (p < .05). The distance of 13-step and 17-step run-up is 22.70±1.06 – 30.61±1.48 meters. The curve of the run-up speed during the last 25 meters of whole run-up process indicated that the speed became faster associated with the increasing of running distance. The sectional velocity just before taking off (0– 5 meters) was the fastest (8.93± 0.43 m/s). Each sectional velocity of the last 25 meters of whole run-up process was significantly correlated with the performance of long jump (p < .05). The coefficients were .58, .64, .76, .75, and .67 respectively (from 25 meters to touch-abroad). The average utilization rate of the run-up speed is 94.80±4.2% which did not show significant relation with the performance of long jump (p > .05). The conclusion of this study is as follows: The velocity of each section of the run-up process is related to the performance of long jump; therefore, the focal point of run-up training should be emphasized on the accelerative skills. The participants’ curve of run-up velocity and utilization rate of the run-up speed are inconsistent with researcher’s expectation, which needs to be further explored if those findings resulted from experimental design.