There are two stages to design the experiment of Herman grid illusion. The main purpose of the study is to detect the percentage relationship of grids and aisles. And we try to understand the different illusive effect of changing colors. The results are below: The percentage relationship of grids and aisles is 1 to 0.75. It means that the square areas of them are four to three then the gray point will show. From the analysis of variance we find that it has significant Hermann grid illusive effect as the colors change because of the value smaller than 0.05. The color ranks of the Hermann grid illusion are black, gray, red, purpleblue, green, blue, purple, yellowred, purple, greenyellow, bluegreen, and yellow. The results can apply to interface design or the traffic safety management. We hope that the drivers can raise the correct judgment rate and use the design of interface safety to decrease the illusion or protect from danger.