As the educational technology field moves away from the stand-alone technology course and moves toward integrating technology across e-learning courses, the tools to develop e-learning courseware need to be considered. Courseware for computer application software is very commonly used in many e-learning courses. The purpose of this study focuses on the design and development of web-based courseware for Digital Audio Processing. Following the procedural learning strategies, this study designs, develops, and evaluates the web-based courseware for Digital Audio Processing Course. This study compares five software programs, i.e., Adobe Presenter, Articulate Presenter, PowerCam, Camtasia Studio, and Macromedia Captivate, selected according to the generalized psychomotor domain learning attributes. The results provide useful guidance for developing e-learning courseware.