This study investigated the effects of the baseball seams on flying trajectories and velocities. Standard baseballs, high-low seam baseballs and wide-narrow seam baseballs were pitched with two-seam fastball by using the Jugs pitching machine at 37.5m/s in the indoor bullpen. Two Sony digital cameras (60HZ) were used to collect date and a SIMI motion analysis system was used to analyze parameters such as the horizontal displacement, the vertical displacement and the velocity. The result indicated that first, the high-low seam baseball has the most amount of horizontal displacement (about 0.036 m), and the wide-narrow seam baseball (about 0.031 m) also has a bigger horizontal displacement than the standard baseball (about 0.020 m). Second, the standard baseball has the most amount of vertical displacement (about 0.137 m) than the other two kinds of baseballs. The high-low seam baseball (about 0.128 m) has a bigger vertical displacement than the wide-narrow seam baseball (about 0.123 m). Third, three kinds of baseballs have almost the same velocity curve. The findings suggested that changing the baseball seams would affect both the horizontal and vertical displacements of flying trajectories. In particular, the high-low seam baseball has the most amount of displacement at the horizontal axis compared with the standard baseball. Therefore, the baseball with the changing seam could be used and simulated in a real pitching situation during batting practice, because of automatically changing flying trajectory.