The main purpose is to explore the effectiveness about body characteristics and health fitness between regular and extension female students in one middle-Taiwan university for one 12-week Training. Participants were conveniently chosen from researcher' classes and 50 female students in regular class group and health fitness and 50 female students in extension class group. Both groups were 22-24 years old. Pre-test and post-test were taken before and after 12-week fitness training. Both body characteristics and health fitness, were included in the test. Body characteristics contain body height, body weight, chest girth, wrest girth, hip girth, and health fitness contains aerobic fitness, muscular fitness, flexibility, and body composition. After 12-week training, the results with the analysis of t-test and ANCOVA showed that: (1) There were significant differences between pre-test and post-test about body height, chest girth and wrest girth; and there were no significant difference about body weight and hip girth in regular group. (2) There were significant differences between pre-test and post-test about body height and body weight; and there were no significant differences about chest girth, wrest girth and hip girth in extension group. (3) There were no significant differences between pre-test and post-test about sit-ups, push-ups, sit and reach, body composition and 1600m walk in regular group. (4) There were significant differences between pre-test and post-test about sit-ups, pushups and body composition; and there were no significant differences about sit and reach, and 1600m walk in extension group. (5) There were no significant differences about body characteristics and health fitness with ANCOVA analysis in both groups.