Knowledge updates speedily. In order to offer more and more effective, accurate, safe, and high-quality levels of patient nursing care, the professional ability of evidence-based nursing (EBN) becomes the essential ability of nursing staffs. This article described the process of implementation and achievements of EBN in a medical center for example. The methods include holding related books reading group, participating in related contests inside and outside the hospital, connecting available advanced EBN with nursing practices, joining in the monthly conferences of evidence-based medicine, examining and practising EBN clinically, drafting action schemes, revising and setting norm based on evidences, etc. This article was just for sharing experiences of implementation of EBN.