The purpose of reference service evaluation is to understand whether the reference service, including reference processes and reference results, have met the need of patron's reference need to improve and to enhance the quality of reference services. In the virtual reference service environment, service quality relies not only on the resources, and the online interactive interviews, but also the system and technology needed for virtual reference environment. The purpose of this study is to identify the proper reference evaluation indicators for virtual reference service. This study first constructs a virtual reference service environment. A virtual reference room includes features of voice chat, text communication, and co-browsing web pages. Nine volunteer librarians and thirty patrons with genuine reference questions are recruited for the study to simulate the real online reference situation. By analyzing the different features of traditional and virtual reference services, three dimensions of evaluation indicators are proposed, including: (a) traditional resource-oriented, for example, the accuracy and immediacy of answers; (b) need-oriented, that is, through the interview process, the librarians are aware of the problems of the patrons, and the patrons understand the instructions of the librarians, and whether the interaction process satisfied, and (c) the online virtual reference service system per se, particularly, the ease of use of the system interface. Pre- and postquestionnaires for the patron are designed. The volunteer librarians are requested to take journals for each reference interview. This paper analyzes the patrons' pre- and post- questionnaires as well as librarians’ journals. The evaluation results are reported. The framework of three dimensions of virtual reference evaluation indicators are discussed accordingly.