Background and Aims: Issues about long-term care institution are increasingly important in aging society. Therefore, to understand and study the service quality satisfaction in long-term care institutions are also the basis of establishing long-term care resources. The purposes of this study are to validate the construct validity and testify the reliability of the Chinese version of “Service Quality Satisfaction Scale in Nursing Home” with structural equation modeling for assessing of service quality satisfaction in nursing home. Methods: Cross-sectional design was conducted and one hundred and thirty two subjects were investigated with purposive sampling. Afterwards, confirmatory factor analysis with structural equation modeling and SPSS was used to testify the goodness-of-fit of the hypothesis measurement model. Results: The Chinese version of Service Quality Satisfaction Scale in Nursing Home, consisting of 12 items within a four-factor model, was confirmed with adequate reliability and validity. Four factors are included: Comfort and Cleanliness, Nursing, Food and Nutrition, and Facility for Caring Services. The most satisfaction in service quality amongst was nursing. The score for four subscales are statistically significant among different background groups, indicating our scale has good construct validity.Conclusion and recommendations: The confirmatory factor analysis conducted by structural equation model indicated that: 1. the scale with 12 items was confirmed as a four-factor structure as: comfort and cleanliness, nursing, food, and facility care and services. 2. There are significant differences between demographic background groups. 3. The scale had an appropriate reliability and validity. Health care managers in nursing home can utilize these subscales indicators to evaluate and improve the service quality in long-term care institutions. According to these results, we suggest that the further studies will analyze and utilize this developed scale and its research results to provide and promote the quality of service in long-term care institution.