Purpose: Understand college students' physical fitness, dietary and sleep status. Methods: We designed a stratified random sampling in 80 universities and randomly selected for each year, from freshman year to senior year, 50 males and 50 females between 19- and 23-year-old, for a total of 400 students per college. We developed a survey form including both a questionnaire survey on college students' physical activity and the results of college students on physical fitness tests. We obtained 28977 valid forms. Results: 1) College students improved on sit-ups per minute, but regressed in sit and reach, standing broad jump, 1,600 m or 800 m walk- run tests. 2) The majority of students "always" and "usually" eating breakfast. The majority of college students "always" and "usually" ate everyday normal quantities of food, three times a day, at regular time. Over half of college students "sometimes" and "never" ate fiber-rich food during their meals. Over half of college students "sometimes" and "never" ate daily the five main food categories. Half of college students "sometimes" and "never" drank at least 1,500 cc a day. The majority of college students perceived their own sleep quality as "average" to "very bad". The majority of college students slept on average 6-8 hours a day. There were significant correlation with BMI, flexibility, cardiovascular endurance, and parts of dietary in male students. There were significant correlation with BMI, flexibility, muscular strength and endurance, cardiovascular endurance, and parts of dietary or sleep in female students. Conclusions: 1) College students' muscular strength and endurance have improved, but their flexibility, power and cardiovascular endurance got worse. 2) The dietary of college students still needs to improve. College students' sleep length is sufficient but their sleep quality still requires improvements. 3) There were significant correlation with physical fitness and parts of dietary or sleep in college students.