In recent years the rapid development of Internet has offered us with a diversification of reading choices. The web pages are able to transmit large numbers of real-time information to users through the Internet platform. However, as the current composition and layout of web page design are lacked of specific and precise research guidelines for references, they often cause the designers to use the intuitive customary habit in designing and this prone to missing the opportunity to seize users' attention. Therefore, how to lay out the web pages well and convey the integrated messages to users clearly is an important issue in web page design.This study retrieved 78 outstanding samples from Design Charts website to serve as the analytical samples. The impact of emotional awareness and preferences on different web page compositions on users were measured, and proposals were made on ”main visual position”, ”graphic and text proportion”, ”blank capacity”, ”overall composition layout”, and other layout elements were cross-examined through a content analysis and a questionnaire survey to work as the design principles of web page design. The study discovered that: (1) The composition with ”main visual position at the screen center” was able to enhance the ”special feeling” of design collage students on screen image effectively. (2) The composition proportion of ”diagram bigger than text” was able to improve the ”easy feeling” of users on reading the screen. (3) A blank space of ”40%~60%” was able to upgrade the ”simplicity feeling” of users while watching the screen, but there was still room for further exploration on the effectiveness. (4) The overall composition layout of ”main visual position at the center, diagram bigger than text, and blank space larger than 40%” were able to improve the ”popular sense” and ”love feeling” of users on the overall layout of web pages.