This study uses technology readiness and perceived usefulness and perceived ease of use of acceptance model as independent variables and user's intention of bike sports participation toward the use of a GPS Satellite Navigation System as a dependent variable to investigate the user's inclination and behavior toward using a GPS Satellite Navigation System with a questionnaire survey. In total, 210 copies of the questionnaire were issued with a 100% response rate and 200 valid copies, accounting for 95.2%. Research results indicate that (1) Technology readiness has a significant positive influence on the perceived usefulness; (2) Technology readiness has no significant positive influence on user's intention; (3) Technology readiness has a significant positive influence on the perceived ease of use; (4) Perceived ease of use has a significant positive influence on perceived usefulness; (5) Perceived usefulness has a significant positive influence on user's intention; and (6) Perceived ease of use has a significant positive influence on user's intention.