Purpose: This study utilized a servo-motor controlled platform to adjust the putter speed and to develop a new golf putter testing system (GPTS) that can simulate putting movements. Actual tests were conducted to validate the reliability and validity of the system. Methods: A servo motor, photocells, connection rod, and other structures to simulate putting movements were integrated by a testing system using the LabVIEW instrument control interface. Two high-speed cameras (240 fps) were used to obtain the three-dimensional putting parameters of a female college golfer. The acquired parameters were then inputted into GPTS for reliability and validity testing. The ball movements in specific putting scenarios were simulated by GPTS. The reliability of the repeated putting movements was assessed by statistical analysis through CV (coefficient of relative variability). The validity analysis was employed to compare the human putting distances and the GPTS simulated ball rolling distances with independent sample t-test. Results: In the reliability test, the repeated putting CV values of three putting speeds in GPTS were all below 1%. In the validity test, the human putting movement and the GPTS simulated ball rolling distances were consistent, without significant differences. Conclusions: GPTS has good reliability and validity, and is able to simulate human putting movements. In the future, this system can be used to test the characteristics of different putters, and facilitate the diagnosis of golfers' putting techniques.