Purpose: This study was to explore student nurses' transitions of spirituality and applying spiritual nursing care in practicing hospice care. Material and Method: Twoyear baccalaureate nursing programs students, who had chosen the course of hospice nursing care and participated for hospice clinical practice, were interviewed. Ritchie and Spencer's (1994) framework of thematic analysis with software Nvivo 9 was used to analyze the interview data. Results: The difficulties of spiritual care realized by student nurses included: hurrying to do something, insensitivity in communication, lack of clinical experiences, religious factors and self-limitation. The factors to promote spiritual care by self-awareness of student nurses were: ambient stimulation, good learning models, the self-reflection of nursing attitude and complement each other in peers. In transitions of spirituality for student nurses included: living in the real moments, love and devotion for parents in time, self-respect and having courage to face life, learning thanksgiving and forgiveness, re-awareness of death and nursing value. Conclusions: This study displayed the reflection of spiritual care and self-awareness of spirituality for student nurses in palliative clinical practice. It will provide the guideline of life education, spiritual, and hospice nursing care and clinical practice.