The purpose of this study was to investigate the effect of long-term pitch on lower extremity kinematics of women’s softball pitchers.7collegate softball pitchers were recruited to pitch most 10 innings simulated game in biomechanics lab. Motion analysis system was captured lower extremity kinematics on pitching acceleration phase. All parameters were examined by nonparametric method (wilcoxon signed rank test)between pre(first inning) and post(last inning)pitching movement. The result was displayed the post stride leg knee flexion angle was significant smaller than pre test on leg landing moment. And the post ankle eversion angle also smaller than pre test significantly on ball release moment. However, the ROM and angular velocity was no significant in pre-post test or any moment. Concluded: The stride leg landing movement was more stiffness land after long-term pitch. And the ankle was decrease the sustain angle and angular velocity to maintain the knee and hip joint stability in order to facilitate the ball release.