This study is to understand the influence of the impact to human body when riding folding bike on different deceleration road. The measuring Methods:by LabVIEW Object-Oriented Programming, putting the accelerometer on the front fork and the seventh cervical vertebra (C7) to test the acceleration and total acceleration. The main instruments for the experiment were LabVIEW Object-Oriented Programming, 2 sets of the accelerometer (Biopac TSD 109F 50G), 1 RTFS contained infrared shutter (E3Z-T61), Ni WIFI analog-digital set-top box (WLS-9163), and NI analog-digital set-top box (USB-6211). 10 college students with riding experiences were the participants. Each of them rode across 50 meters of different obstacle 3 times to collect the biggest acceleration as the sample. The experiment field was 50 meters with one RTFS contained infrared shutter set up at 1 meter before the obstacle road to acquire the passing time and speed parameters, which was transmitted to LabVIEW Object-Oriented Programming through NI WIFI analog-digital set-top box. A statistic test was performed with dependent sample T-test, the prominent standardα=.05. The Results: front fork- the impact index of the accelerometer has prominent differences. The impact index was obvious because there was no cushion for damping. C7Y axis and C7 total acceleration- when human body encounter impact, the test respondents support the upper body with the arms to resist the impact and further damping. The Conclusions: front fork- the impact index of the accelerometer had prominent differences, reaching .05 standard. As there was no cushion for damping, the impact index was high. C7Y axis and C7 total acceleration- there had no obvious differences in impact index. The main reason was that the test respondents would support the upper body with the arm strength to resist the impact and feather damping.