The progress of information technology is rapid. It is gradually included in the nursing care practice. However, it might have great impact on traditional nursing practice. In this study we explored the relation among acceptance, use intention and satisfaction of mobile nursing station (MNS). Study subjects are nurses, from a medical center and its branch in central Taiwan, who had a rank of N (or above) and have operated the MNS for at least three months. The questionnaire covers personal information, acceptance of the MNS (including cognition of usefulness, cognition of ease use, user attitudes, and technology anxiety), use intention and satisfaction. The results show that the cognition of usefulness and attitudes of nurses have positive influence on the use intention, but technological anxiety has negative influence on the use intention. Meanwhile, the use intention has positive influence on use satisfaction. The results also show that users who practiced more frequently before using the MNS will have higher acceptance, use intention and satisfaction.