Messages transmitted through mass media impact audiences, especially those transmitted through television programs. Domestic and foreign research has demonstrated that people draw on scientific knowledge through mass media. In recent years, the Taiwan government has recognized the importance of disseminating science-related knowledge and has promoted the Development of Taiwan Science Communication Industry Project－Media Pilot Project. One of the purposes is to upgrade the quality and the quantity of science programs to better disseminate science-related knowledge to the public. Through the broadcasting of cable, wireless and digital television programs, it is expected that the public's science-related knowledge and literacy will be elevated. To understand if these objectives are being achieved, it is important to conduct a survey of audiences to understand their feedback. This is the aim of this study. Four evaluation categories were developed from a review of the literature. Two focus groups made up of those who watched these programs were conducted. The results demonstrated that there are several indicators that affect audience evaluation of science programs, including program theme and content, format, structure, flow, narration, voiceovers, subtitles, cinematography, visual quality, authenticity, appropriate entertainment level and attainment of knowledge. In addition, this study found variations among age groups in terms of their evaluation of program quality. For example, younger audiences place more importance on production techniques. Thus, it is suggested that production companies producing popular science programs pay attention to the demographics of their target audiences when creating new programs.