PURPOSE: The aim of this study was to investigate the difference health-related fitness of students from 2012 in Lee-Ming Institute of Technology. Subjects had 787 students and include 487 males and 300 females. So as to understands fitness situation of students and the teaching reference. METHOD: The test was based on “College Students Fitness Passport” were use on PE class. This contents were include BMI, standing long jump, sit and reach flexibility test, one-minute sit-up, 800m running for female and 1600m running for male The investigated values were analyzed by Descriptive Statistics. RESULTS: 1) All health-related fitness tests on standing long jump were poor performance for males and females. 2) On the 800/1600 meter running have to improved need. CONCLUSION: The next teaching in PE course and the PE teacher was to promote in explosion strength and cardiopulmonary endurance that were be improved students physical ability.