Purposes: To investigate the training effects of Mach drills on physical fitness quotient in high school students for 12 weeks. Methods: The participants were 200 high school students. After the PFQ pre-test, participants were divided into experimental (n = 104) and control groups (n = 96). In experimental group, participants were taught the Mach drills for 12 weeks. In control group, participants were only taught normal physical education classes. All the participants were accepted PFQ tests after intervention. Raw data were transferred to scores by PFQ norms and using the independent t test to compare the difference between experimental and control groups (pretest minus posttest). Using the independent t test to compare the differences of all grades. Results: The experimental group was significant higher than control group in PFQ, HPFQ, SPFQ, muscular fitness scores, flexibility scores, cardiorespiratory fitness scores, agility scores, coordination scores, speed scores and power scores (p < .05). The body composition, balance and reaction scores were no difference between groups. After the Mach drills training, the items of physical fitness were decreased with ages (8 items in freshman, 5 items in sophomore, 2 items in junior). Conclusion: The Mach drills training for 12 weeks increased the PFQ of the senior high students.