Background: The loss of nursing manpower in the Emergency Room (ER) was increasing gradually. To understand the job stress of nurses working in the emergency department could reduce the working pressure and relieve some stress. Purposes: The purposes of this research were to understand the nurses’ working stress of emergency rooom and analyzing the influencing factors. Methods: Regional hospitals in northern Taiwan of 100 nurses were chosen as sample size. This research started from September 1, 2014, and ended to January 31, 2015. This is a cross-sectional design. A self-developed job stress structured questionnaires for ER nurses were developed. SPSS 21.0 software was used to analyze the data. Observer’s reliability was 0.8. Experts’ validity and content validity were used. Results: The findings showed the total average score of job stress was 3.34. Each dimension of job stress including the stress of disease care, stress of the colleague relationship, stress of clinical ladder system, stress of administrative managers, and stress of working environment, the average score were 3.57, 3.29, 3.34, 3.62,and 3.10 Individual factors, the family factors and the work factors all have significant differences with the job stress in this study. The regression analysis results revealed the sex, the family support and the hospital levels could explain 48.4% amounts of variation; The sex, family support, hospital levels, religious belief, salary, title of the job, clinical ladder system, education levels, children's number, status of health, marital situation, age, family pattern and the years of service may explain 71.3% total amounts of variation. Conclusions/Implications for Practice: This finding showed that the sex, family support, the hospital levels of the emergency department nurses were significant difference on the scores of job stress. From this research, we hope we can understand the working stress in ER nurses and help them to relieve pressure, in order to elevate the quality of nursing care.