Evidence-based nursing (EBN) is a scientifi c method promoted worldwide for solving problems related to clinical nursing practice. Educational training courses incorporating EBN have been used to upgrade the capacity of evidence-based nursing care. The purpose of this study was to understand the effects of studied hospital 3 sessions of EBN training program, the nurses' awareness of EBN knowledge and the application in clinical sitting, and to provide specific recommendations based on the results. The content-analysis method was used to explore the data and analyzed barriers and facilitators based on Joanna Briggs Institute evidenced-based model. The results of this study indicated 34 EBN topics were generated after training program; of 31 EBN topics were completed EBN 5A steps, 25 topics were submitted abstracts and accepted in the conference, including 19 oral and 6 poster presentations, and 13 topics have wined the EBN rewards by different government funded institutions. Furthermore, the result of a EBN topic was integrated into the clinical nursing standard in the studied hospital and disseminated into the daily activity of nurses through the nursing informatics systems. Four EBN topics were published in the peer-reviewed journals within two years. The fi ndings of this study could provide recommendations and strategies of future designing the EBN training program for clinical nurses and as a reference for the nursing researchers and educators.