本院供應中心主要業務為處理手術器械，每天到期器械盤包過多，重新滅菌造成人力、物力、時間與成本之浪費。以病人安全為前提下，本研究目的主要在比較延長手術器械盤包儲存期限是否會增加盤包內長菌率，採調查研究設計。於2015年4月15日至2015年6月5日共2個月期間，以某醫學中心供應中心手術器械為調查母群體，每天從過期未用之雙層棉布包裝的手術器械盤包中抽樣30-38包，共分14組採樣，總樣本數450包；於滅菌效期到期日起超過第1天、第2天至第14天，每天拆包進行生物細菌培養，以瞭解手術器械盤包在不同保存期限下盤包之長菌率。研究結果顯示14組均沒有長菌，研究建議可進一步以嚴謹的實驗設計驗證將雙層棉布包裝之手術器械盤包有效期限從14天延長至28天之可行性。

Expired sterilized surgical instruments must be repackaged every day, which consumes manpower, time, and resources while re-dishing, re-sterilizing, and re-packing in CSR. This study evaluated the bacterial growth rate of sterilized surgical instrument packing to explore the potential for prolonging the validity period of sterilized instruments. Between April 15, 2015, and June 5, 2015, samples from overdue double-cloth surgical instrument tray packages wrapped in 30-38packs daily, A total of 14 sets of sampling 450 package. in sterile period of limitation maturities of more than 1 day, 2 days, and 14 days and unpacking every day biological bacterial culture. Results revealed no bacterial growth in the 14 groups, suggesting that further validation can be extended up to the 28-day period.