目標：細菌為室內空氣品質管理法中醫療機構的管制項目之一，公共空間細菌量檢測須以培養方式取得，尚無直讀儀器用於較快速的巡檢作業。本研究評估微生物螢光分析法作為快速巡檢工具之可行性與適宜性，針對細菌超標醫院建立可快速篩檢室內空間細菌濃度分布的輔助工具。方法：在8處列管醫院的管制空間、空調機房內及外氣引入口，以微生物螢光檢測儀與可培養性細菌採樣器進行平行採樣，其中可培養性細菌的採樣及分析，乃依照環檢所的標準檢測方法進行。結果：就檢測儀在高細菌濃度下的表現，當測得螢光係數大於2.82 V/s時，有極大機會細菌濃度可能高於1,500 CFU/m^3的法規限值。結論：對於已知細菌超標的醫院，可利用螢光檢測儀作為輔助，研判疑似污染源位置之污染程度，快速確認細菌濃度空間分佈潛勢並對可能超標區域進行改善，其結果應具有一定程度的參考價值。待累積更多比對數據後，可利用巡檢的螢光係數來推估細菌濃度，以利醫療機構即時現場評估室內空間細菌的可能分布。

Objectives: In accordance with indoor air quality management regulations, the bacteria concentrations of public spaces in healthcare facilities are determined by using microbial cultures, a method that is time-consuming and inefficient. This study examines the feasibility and applicability of using a semi-portable fluorescence microbe sensor in hospital settings to quickly obtain the potential spatial distribution of airborne bacteria. Methods: Eight hospitals were selected as sampling sites. Both the fluorescence microbe sensor and microbial culture of air samplers were used. Parallel sampling was performed in hospital areas accessible to the public, air conditioning facilities, and fresh air inlets. Results: Fluorescence intensities obtained by microbe fluorescence detection were found to be highly correlated to the bacterial concentrations at 21 sampling locations. When the measured fluorescence intensity is higher than 2.82 V/s, the estimated bacterial concentration has a high probability of exceeding 1,500 CFU/m^3, which is the maximum legal limit. Conclusions: In hospitals where levels of bacteria exceed the legal threshold, the microbe sensor can be used as a fast screening tool to identify the potential spatial distribution of bacteria in real time. Upon completing further studies and comparing more data, using fluorescence intensity to estimate bacteria concentrations is expected to become a more feasible and effective method of monitoring indoor air quality in hospitals.