The hotel industry is a critical business in the tourism industry and important indicator of tourism industry development. Rapid growth of tourists in Taiwan results in an increase of tourist hotels. With the constant growth, how to maintain annual internal balance and stability of the accommodation market of tourist hotels is an important issue. Seasonality is a significant characteristic of the tourism industry and one of the most important factors of related business operations in the industry. By a structural time series model, this study analyzes accommodation demand seasonality of tourist hotels in Taiwan and validates dynamic characteristics of seasonality of accommodation demand of tourist hotels in Taiwan. Through regression analysis, it explores the relationship between accommodation demand seasonality and customer structure. According to empirical results, seasonality of accommodation demand of tourist hotels in seven areas is significant and random. Seasonal factors of accommodation demand of tourist hotels in different areas result in different sources of tourists. Thus, customer structure of tourists in different areas varies. Tourist hotels in different areas should cope with accommodation demand seasonality by different strategies and methods. Regarding seasonality, tourist hotels can adopt a price difference strategy, horizontal alliances or hold activities, conferences and exhibitions with local governments to increase the customers and enhance occupancy rates of hotels.