The aim of this study was to investigate the effect of mozart music on releasing stress. The research used physiological parameters, heart rate variability (HRV) parameters, and electroencephalogram (EEG) signal as stress indicators. Before the experiment, we used physiological monitor to measure the subject's physiological parameters such as systolic blood pressure, diastolic blood pressure, and heart rate. HRV analyzer was to measure the subject's HRV parameters, such as SDNN, RMSSD, total power, low frequency power, high-frequency power, normalized low-frequency power, normalized high-frequency power and low / high-frequency power ratio and EEG device to measure the subject's EEG signal, such as θ, α, middle β (Μ−β), high β (H-β), γ wave. The objects were divided into control group and experimental groups of mozart music K448 and KV550. After listening to music for 10 minutes, we measured the objects’ physiological parameters, HRV parameters and EEG signal again. Pair t test was used to compare the differences of physiological parameters, HRV parameters and EEG signal between pretest and posttest in all the three groups. The experimental results showed that there were no obvious significant difference in physiological parameters such as systolic blood pressure, diastolic blood pressure, heart rate in all the there group. In HRV parameters, K448 can increase RMSSD, ln TP, ln LF, ln HF,and KV550 increase RMSSD. In the electroencephalogram (EEG) signal, only the K448 group can induce an decrease in high β wave, but control and KV550 group induce no significant difference in EEG signal between pretest and posttest. This study confirmed that mozart music may act through increasing the parasympathetic activity or decreasing high β wave to cause a relief of stress on human.