:::

詳目顯示

回上一頁
題名:黑鯛第一型類胰島素生長因子基因片段之合成
書刊名:嘉南學報
作者:吳鴻程范晉嘉 引用關係陳泓志張繼堯吳金洌
出版日期:1994
卷期:20
頁次:頁138-147
主題關鍵詞:黑鯛核苷聚合酶連鎖反應類胰島素生長因子DNA核苷引子Black seabreamPolymerase chain reactionInsulin-like growth factor IDNA primer
原始連結:連回原系統網址new window
相關次數:
  • 被引用次數被引用次數:期刊(0) 博士論文(0) 專書(0) 專書論文(0)
  • 排除自我引用排除自我引用:0
  • 共同引用共同引用:0
  • 點閱點閱:91
     依據黑鯛第一型類胰島素生長因子(Insulin-like growth factor I,IGF-1)DNA 序列,合成長度為23個核��酸的S核��引子,19個核��酸的B核��引子,21個 核甘酸的D核��引子和22個核��酸的E核��引子。配對後的核��引子利用Vent 核背聚合�t進行連鎖反應;其反應條件為:解鏈溫度94℃(反應1分鐘)。黏合溫度 65。C(反應2分鐘),合成溫度72℃(反應2分鐘),共重覆35次循環。經核��合�t 連鎖反應後,可生成B區域到D區域201個核��酸DNA片段;B區域到E區域 423個核��酸的DNA片段,S區域到E區域552個核��酸的DNA片段。S區域 到D區域的DNA片段,則在解鏈溫度94℃(反應30秒),黏合溫度54℃(反應30 秒),合成溫度74。C(反應30秒),共進行35次循環的條件下可生成330個核��酸 之DNA片段。
     Four oligonuclotide primers, primer S (23 bases), primeer B (19 bases), primer D (21 bases)and primer E (22 bases), were synthesized according to the black seabream (Acanthopagrus schegeli) insulin-like growth factor I (IGF-I) DNA sequence to amplify the ICF-I DNA fragments.The results found that primeer S, primer E produced 552 base pairs ICF-I DNA fragment, primer B, primer D producted 201 base pairs IGF-I DNA fragment, and primer B, primer D produced 423 base pairs IGF-I fragment at denatured 1 minute at 94℃, annealized 2 minutes at 65 ℃, and extended 2 minutes at 72℃ by polymerase chain reaction. Primer S and primer D producted 330 base pairs IGF-I DNA fragment at denatured 30 seconds at 94℃, annealized 30 seconds at 54℃ and extended 30 seconds at 74℃ by polymerase chain reaction.
期刊論文
1.Baker, J.、Lin, J.-P.、Robertson, E. J.、Efstratiadis, A.(1993)。Role of insulin-like growth factors in embryonic and postnatal growth。Cell,75,73。  new window
2.Kikuchi, K.、Buonomo, F. C.、Kajimoto, Y.、Rotwein, P.(1991)。Expression of insulin-like growth factor-1 during chicken development。Endocrinol.,128,1323。  new window
3.Salmon, W. D. Jr.、Daughaday, W. H.(1957)。A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro。J. Lab. Clin. Med.,49,825。  new window
4.Byatt, J. C.、Staten, N. R.、Schmuke, J. J.、Buonomo, F. C.、Galosy, S.、Curan, D. F.、Krive, G. G.、Collier, R. J.(1991)。Stimulation of body weight gain of the mature female rat by bovine GH and bovine placental lactogen。J. Endocrinol.,130,11。  new window
5.Duan, C.、Hirano, T.(1990)。Stimulation of 35S-sulfate uptake by mammalian insulin-like growth factors I and II in cultured cartilages of the Japanese eel。Anguilla japonica. J. Exp. Zool.,256,347。  new window
6.Saiki, R. K.、Gelfand, D. H.、Stoffel, S.、Scharf, S. J.、Higuchi, R.、Horn, G. T.、Mullis, K. B.、Erlich, H. A.(1988)。Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase。Science,239,487。  new window
7.Bayne, M. L.、Applebaum, J.、Underwood, D.、Chicchi, G. G.、Green, B. G.、Hayes, N. S.、Cascieri, M. A.(1989)。The C Region of Human Insulin-like Growth Factor (IGF) I is Required for High Affinity Binding to the Type I IGF Receptor。J. Biol. Chem.,264,11004。  new window
學位論文
1.陳泓志(1994)。黑鯛類胰島素生長因子基因之選殖與定序(碩士論文)。國立海洋大學。  延伸查詢new window
圖書
1.Innis, Michael A.、Gelfand, David H.、Sninsky, John J.、White, Thomas J.(1991)。PCR Protocols: A Guide to Methods and Applications。New York:Academic press。  new window
2.Mcpherson, M. J.、Quirke, P.、Taylor, G. R.(1989)。PCR: A Practical Approach。New York. USA:IRL press。  new window
 
 
 
 
第一頁 上一頁 下一頁 最後一頁 top
:::
無相關期刊論文
 
無相關博士論文
 
無相關書籍
 
無相關著作
 
QR Code
QRCODE