This study was to understand the turnover intention and its affecting factors on agricultural extension staff in Farmers’ Association. The data were obtained from 950 answered questionnaire among totaled 1,796 extension staff of Farmers’ Associations(FAs) in Taiwan. The result of path analysis for collected data indicated that the most important and the most direct factor affecting turnover intention of FAS’ extension staff is job satisfaction and organizational commitment, while other job alternative and role-model pressure also showed significantly direct effect. The effect of role-model pressure also is pass through the two intermediated variables, that is job satisfaction and organizational commitment, to affect the turnover intention of FAS’ extension staff. The indirect effect of job involvement and job guarantee is pass through the job satisfaction and organizational commitment on the turnover intention of FAS' extension staff. The result also indicated that the service years and education level was not showing the expected effect. The reason is that there is rather concentrated binomial distribution of the two variables, and the small variation have been omitted and didn’t showed any expected effect.