The purpose of this study was to compare with the extensors and flexors for muscle strength of knees between (1) the dominant and non-dominant, (2) the subjects of injured and uninjured, And evaluated the rates between flexors and extensors (H/Q) in the college female soccer players. 18 subjects (age=19.2b1.3 yrs., ht.=160.4 3.5cm, wt.=53.9 4.7kg.) used Merac Isokinetic Dynamometer to test muscle strength of knees at the speeds of 60 deg/sec, 180 deg/sec and 300 deg/sec. Results indicated that the dominant were significantly (p<0.05) better than the non-dominant for knees extensor peak torque at the speed of 180 deg/sec, which expressed that the players over using the dominant with middle speed to passing within the thirty meters. The contractions of middle and fast speed (65-79%) was higher than slow (49-52%) on the H/Q ratios. Besides, the subjects of injured was 85-88% to the uninjured on the muscle strength of knees, it meant that the relations between the muscle strength and injured. Conclusions: It can keep the balance on the muscle strength of knees as reinforcing the training of non-dominant to female soccer players. The subjects who has injured must strengthen the muscle strength training to avoid getting the sporting injury again.