Extreme temperature and humidity in workplace can significantly influence the results of environmental monitoring. In this study, dynamic standard gas generation system combined with activated charcoal tubes were used to investigate the temperature and humidity effects of six most widely used industrial solvents including toluene, xylene, acetone, methyl iso-butyl ketone, ethyl acetate at the level of 0.5, 1 and 2 PEL (Permissible Exposure limits). The results showed no significant differences between high (80%) and low (40%) humidity for test solvents except Acetone and Ethyl Acetate, which showed lower recoveries at high humidity. The recoveries rates at high temperature (30 ℃ ) are higher than low temperature (20 ℃ ). In general, the recoveries of six mixed solvents in the tested conditions (0.5/1/2 PEL, 20 ℃ /30 ℃, and 40%/80%) were all greater than 80%. Therefore, under normal working conditions, the temperature and humidity effects are not significant.