This article briefly analyze opportunities and challenges of ecological civilization construction in Zhenjiang in order to lay a solid theoretical support and guidance for ecological civilization construction in Zhenjiang. Opportunities of ecological civilization construction in Zhenjiang include good natural endowment,experimentation area of ecological civilization in South of Jiangsu,the only comprehensive reform pilot for ecological civilization construction in Jiangsu,the first batch of first demonstration area for ecological civilization in China,demonstration area of national ecological civilization construction,the leading of low-carbon city building in China,the establish and implement the strategy of eco-city and eco-leading,the construction of the first Eco-cloud in China,and high attention of country on ecological civilization construction. Challenges of ecological civilization construction in Zhenjiang include ecological environment facing multiple challenges,sustainable development facing multiple bottlenecks,the not perfect of ecological system,the lack of atmosphere and mechanism for the public participation in ecological civilization construction,the weak of ecological civilization consciousness for whole society,the crossed function of multiple departments,the indifferent environmental enforcement and poor regulation,the mismanagement and inefficiency of systems for ecological civilization construction,the varied and non-uniform for index system of ecological civilization construction,and the need enhanced investment intensity for ecological civilization construction.