In the study of Shakespearean tragedies, affected by Aristotle’s theory of "character flaw",most critics, including A. C. Bradley, have preferred to attribute the causes of tragedies to the character of the protagonists, and thereby have ignored and concealed the gender feature of the female protagonists. Given the roles of the women in the Shakespeare’s classic tragedies, the paper will focus upon the females’ transgressive gender feature and behaviors which go against the mainstream values of female gender expectations in Renaissance, including cases of Volumnia—a mother in Coriolanus, Lady Macbeth—a wife in Macbeth and Cleopatra—a queen in Antony and Cleopatra for discussion. All of them fall into the category of the female manipulator behind the males protagonists. The paper argues that the transgressive gender feature of the female subject in Renaissance is socially invested, culturally constructed and not naturally invested. Under the dominance of patriarchy and phallocentrism, the cause of the tragedy is mainly due to the transgressive gender feature of the women, which violates the convention of the traditional gender roles in the Renaissance society. In this sense, Shakespeare tragedies, are the tragedies which result from the clashing and contradiction between the socially invested gender feature limitations and the female’s innate desire to pursue power, equality, freedom and spiritual independence.