The present study included two experiments. In experiment 1, we used the pictures of faces displaying emotion as the experimental materials and adopted the dot-probe paradigm to study the attentional bias characteristics of subjects with high and low self-esteem. In experiment 2, we adopted the repetition priming paradigm and dot-probe paradigm to explore the effects of affective priming on attentional bias of individuals with low self-esteem. We can conclude that individuals with low self-esteem tend to pay more attention to angry faces, thus indicating a negative bias in low self-esteem subjects; Affective priming has a significant effect on attentional bias of low self-esteem individuals.Under positive affective priming condition, the negative bias of low self-esteem individuals is improved obviously.Under the condition of negative affective priming, the attentional bias effect of individuals with low self-esteem is stronger than that under controlled conditions.Negative affective priming increased the negative attentional bias of individuals with low self-esteem.