Discourse of post-human subject is formed in the process and context of of reflecting on and criticizing the discourse of modern subject. In this regard,the"Cyborg"discourse of subject,informationalism discourse of subject and"universal-vitality"discourse of subject can be seen as the representative forms of discourse of post-human subject in Western countries. When it comes to the reflections on problems related to solipsism,autonomy and modern subject of possession,discourse of post-human subject has made some achievements. However,there are still some limitations which are respectively the subject concept of adherent entity,the relationship mode between the subject and the object,residual anthropocentrism and so on. This paper has put forward the"mediated subjectivity"discourse which aims at providing a new explanation for post-human subject. Mediated subjectivity discourse takes the mediated "Cyborg"life forms as the material basis. The mediated "Cyborg"is in the basic structure as"individual( body-consciousness)-media-identity". Mediated subjectivity is specially presented as active connection,inviting,gathering,tolerance,interacting with the outside world,joint,infusing and other features. Furthermore,mediated subjectivity also practically realizes the communication between the subject and the world,as well as the existing forms of posthuman subject which are intertwined and infused with one another.