In 1991, while the establishment of the National Museum of Marine Biology and Aquarium (NMMBA) was still in its planning stage, the project was challenged by a drastic cut down of budget and staff number from the government. Dr. Lee-Shing Fang, the founder of NMMBA, decided to seek resources from private sectors to complete the partially build project. He had designed the first Build-Operate-Transfer (BOT) case in culture-education field of the world. By applying a strategy of subletting the profitable sections of the partial finished museum, such as the aquarium, the souvenir shop, the restaurant...etc. to private sector, the museum was able to channel the commercially earned money to finish the remaining project, which is the third pavilion (waters of the world), the visitor center and the Pacific ocean square. This paper is written from an interview with Dr. Fang to explore how the decision was made, how the plan was designed, and what are the advantages and disadvantages of this operation model. It revealed that in this project, the private sector had taken care of daily museum-aquarium operation market promoting and visitor services. This allowed the museum curators to focus on higher level museum works, such as research, public education, special exhibitions, display renovation, intra-museum collaboration, developing strategy etc. There is certain conflict of interest between the public and private sector. However, overall speaking, this is a mutual beneficial cooperation case. The structure is a valuable model for operation of museums. Their experience is also valuable for non-profit organizations, such as museum, to maximize their capability of public service while maintain balance with the interest of collaboration private sectors.