When the National Government (Kuomin Government) controlled most of the territory in China, Guangdong and Guangxi provinces, with the appeal for sharing power between separated governments, still maintained quasi-independence from the Nanjing Central Government by organizing the Southwestern Executive Branch of the Kuomintang and the Southwestern Political Affairs Committee of the National Government, In 1936, the long-standing standoff between the Nanjing Government and the Southwestern National Government showed a dramatic change. Firstly, the Yuehan railway was set to go into operation, which would greatly benefit the Central Government in entering Guangdong. Secondly, Hu Hanmin's sudden death of cerebral haemorrhage deprived Guangdong and Guangxi of their important political patron. The powerful leaders of the Guangdong and Guangxi Clique, including Chen Jitang, Li Zongren and Bai Chongxi, opposed Japan to increase military forces in the North of China and used this issue as a pretext to urge the government to fight against Japan immediately. It was called the "Guangdong-Guangxi Incident" or the "June 1 Incident." The research drew reference mainly from the Chiang Kai-shek Collections and was supplemented with the Memoirs of Chen Cheng: the North Expedition and the National Unification and the Chen Cheng Correspondence Collection with Family newly published by the Academia Historica and the Diary of Wang Tzu-chuang published by the institute of Modern History, Academia Sinica. This research hopes to gain better understanding of Chiang Kai-shek's attitude toward the Guangdong and Guangxi Clique during the Guangdong-Guangxi Incident. The research tried to analyze different policies Chiang Kai-shek adopted towards Guangdong and Guangxi respectively at the beginning of the incident. And then the research explained why Chiang changed his attitude toward the Guangxi Clique dramatically after Chen Jitang's power collapsed. Finally, the research discussed what Chiang and the Guangxi Clique had in mind to end the Guangdong-Guangxi Incident peacefully. The peaceful ending of the incident meant that Chiang Kai-shek was prepared for the challenges from the powerful domestic warlords while maintaining not to launch wars easily. He established the image of being willing to negotiate for peace and was embraced bygood public opinion. This was a sign that he was getting more proficient in political maneuvering.