The purpose of this study was to explore the differences between the daily habits and health status among different cultural groups. In this study, a total of 592 valid subjects from four ethnic groups as indigenous people, Taiwanese, Hakka, mainlander around Kaohsiung City and Pingtung County. The elderly lifestyle questionnaire was adopted as the research tool of this research. Using SPSS13.0 statistic software, a descriptive statistics and chi-square test of independence were applied to analyze the collected data. The level of significance was set at α=.05. The results were as follows: 1. There were no statistically significance for smoking status among different cultural groups. Various ethnic groups have 25% smokers. 2. The aborigines have higher proportion of drinking and eating betel than those of other ethnic groups. 3. The aborigines have higher proportion of being not picky eaters, and other ethnic groups have higher proportion as vegetarian. 4. Four ethnic groups would eat healthy food, but Hakka people have higher proportion of eating healthy food. 5. Hakka people have higher proportion in participating in indoor sports, while the Taiwanese ethnic group has higher proportion of being not taking exercise. 6. The Taiwanese ethnic groups has higher proportion of being insomnia. 7. The indigenous people has higher proportion of being illness than those of other ethnic groups. 8. Hakka people has higher proportion of perceived healthy, while the Taiwanese ethnic group perceived a higher proportion of bad health situation. 9. There were no significant differences among different cultural groups in taking medicine.