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題名:補充大蒜對下坡跑運動後發炎反應、氧化壓力及淋巴球細胞凋亡的影響
作者:羅欣怡 引用關係
作者(外文):Hsin-I Lo
校院名稱:國立體育大學
系所名稱:體育研究所
指導教授:許美智
蘇柏誠
學位類別:博士
出版日期:2010
主題關鍵詞:大蒜下坡跑發炎反應氧化壓力淋巴球細胞凋亡garlicdownhill runninginflammatory responseoxidative stresslymphocyteapoptosis
原始連結:連回原系統網址new window
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本研究主要探討四週的大蒜膠囊的增補對於下坡跑運動後發炎反應、氧化壓力及淋巴球細胞凋亡的影響。本研究採隨機雙盲交叉試驗,並以13位未受訓練健康男性學生為受試者,每位受試者必須完成二階段實驗,分別給予大蒜粉末 (300 mg/顆,約含allicin 3mg;GA組) 或安慰劑(PL組) 增補四週,每日服用5顆膠囊,增補期過後,所有的受試者在跑步機上進行坡度-10 %、強度為60 % VO2max的下坡跑運動60分鐘,於恢復期持續補充膠囊2天,並採集受試者補充前 (Pre-S)、運動前 (Pre-Ex)、運動後 (Post-Ex)及恢復期1 h (Post-1 h)、2 h (Post-2 h)、24 hr (Post-24 h)、48 h (Post-48 h) 及72 h (Post-72 h) 靜脈血液進行血液分析及填寫肌肉痠痛問卷。研究結果顯示,大蒜萃取物的補充對於延遲性肌肉痠痛 (delayed onset muscle soreness; DOMS) 及肌酸激酶 (creatine kinase; CK) 無顯著影響。發炎反應方面,大蒜的補充對於運動後腫瘤壞死因子(tumor necrosis factor-α; TNF-α)、介白素-1β (interleukin-1β; IL-1β) 及介白素-10 (interleukin-10; IL-10)濃度無顯著影響;但GA組可抑制下坡跑運動後介白素-4 (interleukin-4; IL-4)濃度的減少。氧化壓力方面,運動後PL組的8-iso-prostaglandin F2α (8-iso-PGF2α) 濃度顯著高於GA組;GA組的活性氧物質 (reactive oxygen species; ROS) 於運動後1 hr及2 hr顯著低於運動後立即點,但PL組無顯著變化;而穀胱甘肽 (glutathione; GSH) 於下坡跑運動後2 hr的顯著降低,而GA組則於運動後24 hr顯著高於PL組。淋巴球細胞凋亡方面,大蒜的增補可抑制運動後粒線體膜電位 (mitochondrial transmembrane potential; MTP) 的降低及抑制運動後24hr細胞凋亡的發生;此外可抑制caspase 3活性的增加,但對caspase 8及caspase 9無顯著影響。因此,四週大蒜萃取物的增補具有抗發炎反應的潛在性效果,亦可抑制脂質過氧化反應及細胞內氧化壓力的增加,進而抑制下坡跑運動後所造成初期的淋巴球細胞凋亡現象。
The purpose of the present study was to investigate the effect of garlic supplement on inflammatory response, oxidative stress and apoptotic response of lymphocyte after an acute downhill running. The experiment was in randomized, double-blind and cross-over designed. Thirteen untrained healthy male subjects were recruited in this study. All subjects were asked to perform two downhill runs which separated at least five weeks. Subjects took 5 capsules of garlic (300 mg garlic capsule with 3mg of allicin, GA group) or placebo (PL group) each day for 4 weeks. After supplementation, all of the subjects were required to run on a downhill (-10%) treadmill exercise at an initial speed corresponding to 60% of their individual VO2max for 60 min. During recovery period, the capsules were also taken. Venous blood samples were collected pre-supplement (Pre-S), pre-exercise (Pre-Ex) and immediately (Post-Ex), 1 h (Post-1 h), 2 h (Post-2 h), 24 h (Post-24 h), 48 h (Post-48 h) and 72 h (Post-72 h) after the exercise. No effects were found in delayed onset muscle soreness (DOMS) and creatine kinase (CK) levels in the GA group. In inflammatory response, there was no significant difference between or within groups in tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-10 (IL-10).GA group seems to suppress tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) after exercise. The levels of interleukin-4 (IL-4) were inhibited after downhill running in the GA group. In oxidative stress response, the concentration of 8-iso-prostaglandin F2α (8-iso-PGF2α) increased significantly at Post-Ex in PL group compared with the GA group. The levels of reactive oxygen species (ROS) at Post-1h and Post-2 h were lower than Post-Ex in the GA group, however, no change was found in the PL group. Glutathione (GSH) elimination was found at Post-2 h in the two groups; nevertheless, the level of GSH increased significantly at Post-24 h in the GA group compared with the PL group. In lymphocyte apoptosis, the drop in mitochondrial transmembrane potential (MTP) and the percentage of apoptotic cells were inhibited in GA group. The activities of caspase 3 were suppressed in the GA group, whereas no change in caspase 8 and caspase 9 were observed. The results suggested that 4-week garlic extract supplement might potentially inhibit inflammatory, lipid oxidation and oxidative stress after downhill running. In addition, it could suppress the initial stage of lymphocyte apoptosis.
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