100% of Compact Disc-Rewrittable (CD-RW) life testing (or cycliability testing) is time-consuming and costly. Since this kind of testing is a destructive test and need spend 2.5 ~ 3 days/piece. Therefore, the research focus is to derive the intermittent batch sizes (l) and sample sizes (n) in the production line for every cycliability testing of CD-RW. It is noted that sequential test method reduces the sample sizes, n, greatly. A downward specification of sequential tests based on the sequential probability ratio test, risk levels of α andβ (ie., Type I and Type II error), estimated written-counts, t, of CD – RW, and the tolerance, T, of written-count, and the population standard deviation, α, of written-count have been applied to determine the sample sizes, n, for cycliability test of CD-RW. Theoretically, the average number of sample sizes (ANSS) for sequential test required determining the cycliability of CD-RW has also been derived based onα, β, t, T andσ. Furthermore, the degree of freedom in chi-square distribution, (1 –σ) level of confidence and sampling variance S2 have been used to determine the estimated range ofσ2 (ERσ2). An inverse chi-square approximation have also been used to determine the relationship between v = (m – 1), ERσ2 and n at different levels of confidence to determine the batch sizes, l, in a production line.