Driving behavior analysis is a very critical part in traffic safety. To resolve traditional data collection difficulties for analyzing driver behavior, the vehicle driving simulator has been proven a cost effective alternative. Traffic control devices need to be tested under various environments to be installed correctly. The driving simulator provides the best experimental platform for assessing effectiveness of traffic control devices. With virtual reality technology, the driving simulator may well represent all kinds of roadway traffic characteristics. Driver's perception and response to various signs and markings is considered in this study. The data collected in this study indicate that the experimental environment is suitably set and the results are quite reasonable.