The objective of this study was to use the national health-related fitness data of male adults, the age ranging from 25 to 49 years old, including 9 measurement indexes (systolic pressure, diastolic pressure, static heart rate, height, body weight, 30s sit-ups, 60s sit-ups, sit and reach, and cardiorespiratory) The study used 5 years interval as the group to find the optimal age in dividing the group by utilizing hierarchical clustering analysis, and one-way ANOVA. The results showed that the best way to divide group was by adopting the age ranges of first group, second group, third group, forth group, and fifth group, from 25 to 28 years old, 29 to 32 years old, 33 to 37 years old, 38 to 46 years old, and 47 to 49 years old, respectively.