purpose: This study was to investigate the effects of normobaric hypoxia on resting HRV in male college students (n=9) (age: 22±1.5yr; heigh: 174.1±4.4; weight: 72.6±8.19kg) majoring in physical education. We investigated the changes between normoxia (21% O2) and hypoxia (16% O2) and used the time domain analysis index to compaired the different between this two conditions. Methods: electrocardiogram (EKG) signal was taken while the subject was in seat position in a quiet room and breathing the mixed gas (21%O2 + 79%N2 or 16%O2 + 84%N2). The HRV time domain index assessed by the R-R interval which was translated from the EKG signal. Results: Showed that in normobaric normoxia and hypoxia condition. The HRV time domain index were significant decreased in mean R-R interval (845.05±25.79ms & 742.53±61.95ms, P<0.01), RMSSD (53.01±19.96ms & 27.87±9.09ms, P<0.01), PNN50 (26.97±14.63% & 8.39±5.36%, P<0.01), SDNN (83.71±23.97ms & 52.47±18.18ms, P<0.05) and NN50 (58.88±28.17 & 18.66±11.94, P<0.05) but increase in mean HR (81.75±7.16 & 71.78±2bpm, P<0.01). Conclusions: normobaric hypoxia has significantly decrease the HRV time domain indexs and increase resting heart rate.