The purpose of this study was to realize the effect of landscape elements on respondents’ physical and psychological responses. The Conjoint analysis was used to discuss the strength of different landscape elements. Theories of biofeedback and natural benefit on human were discussed. Landscape elements, which include trees, water, and flower, were arranged in 12 siumulated images to test respondents’ response. By the test of Repeated Measure ANOVA, the result shows that different compositions of landscape elements have different effect on physical response (left-brainαwave, Electromyography: EMG, Blood volume pulse amplitude: BVPA). For the psychologicla effect, the four kinds of emotion index (joyful-kind, calm-happy, sinister-confuse, tension-afraid) all have significant differences when showing the respondents with different composition of landscape elements. The Conjoint analysis was used to analyze the strength of different landscape elements. Warm color flowers have significant effect on raising respondents’ left and right-brain α wave, BVPA, and lower their EMG. While cold color flowers lower respondents’ both left and right-brain α wave, EMG and raise their BVPA. When showing the landscape with water, respondents’ right-brain α wave, EMG (EMG), BVPA increase, while the left-brain α Wave decreases. When showing the images with tree, right-brain α wave, EMG, left-branin α wave decreases and higher the BVPA. For psychological effect the warm color flowers decrease the calm-happy/sinister-confuse and increases the joyful and tension-afraid. When showing images with trees the tension-afraid index increases and joyful-kind/calm-happy index decrease. When showing landscapes with water, the joyful-kind and calm happy index increase. From the results of conjoint analysis, the different landscape elements have significant effect on respondents’ physical and psychological effect. The flower colors have most strength effect compare to other landscape elements.