The Response time of Karate upper roundhouse kick consists of two parts: reaction time (RT) and movement time. The reaction time can be divided in to premotor RT and motor RT. This research is focusing on the difference in each time of the movement of the two upper roundhouse kicks, and the relation of each time in the movement of upper roundhouse kick. The subject for ten players, who have own the first three places in Taiwan Karate competitions. In this research, light reaction recorder, foot-switch and Bio-vision EMG were used to record the premotor RT and motor RT. The results point out: 1.Premotor RT of back-leg upper roundhouse kick were faster than front-leg upper roundhouse kick (P<.05). 2.Motor RT of front-leg upper roundhouse kick were faster than back-leg upper roundhouse kick. 3.In the kicking reaction time of front-leg upper roundhouse kick the Premotor RT of superior group is fast than general group (P<.05). 4.In the kicking reaction time of back-leg upper roundhouse kick the Premotor RT of superior group is fast than general group (P<.05). the Premotor RT of reaction time represent an ability in central (P<.05). The Premotor RT of reaction time represent an ability in central information processing. It can be used as a crucial target of assessing and choosing players.