The car following model of bus driver's psycho-physical behavior is the basis of bus rear end collision warning system (RCWS) development and driving safety spacing rule regulation. The threshold equations and parameters of psycho-physical behavior model can also be developed by the bus driving simulator deployment and the simulation analysis. Since the planning and design of simulation experiment scenario is a key element for the success of bus driving simulation analysis, This study planed and designed the driving simulation scenario for developing a bus car following psycho-physical behavior threshold model on freeway straight road section based on the characteristics of bus driving simulator. The results of this study can be a useful basis for developing the bus psycho-physical behavior threshold model in Taiwan.