目的：探討服用魚油與單次激烈運動形成氧化壓力的差異，是否具有加成性的關係，俾使兩者皆能充分發揮對健康的效益。方法：以33位未曾接受過運動訓練男性，分為4組。運動組得接受2次近乎衰竭的間歇運動評估；魚油組每日服用魚油9公克並持續四週。運動前後均抽血檢驗，總抗氧化能力(total antioxidant ability TAA)、超氧離子歧化酶(superoxide dismutase, SOD)、穀胱苷肽總量(total glutathione, t-GSH)、胱苷肽過氧化酶(glutathione peroxidase, GSH-Px)、丙二醛(malondialdehyde, MDA)等。採廣義估計方程式(generalized estimating equations, GE.E.)分析各依變項間差異。結果：運動與魚油不存在交互作用，故其影響具有加成性。魚油組SOD活性較未服明顯多出11.27(11.8%)kU/g-HB(p<.05)：魚油組與運魚組之t-GSH分別明顯增加15.92%及16.07%(p<.05)：除單純運動或魚油分別造成MDA明顯增加0.12及0.27 nmol/ml (p<.05)外，運魚組之MDA亦明顯有加成性增加(p<.05)達35.5%。結論：單次衰竭性激烈運動及單純服用魚油四週後，均造成體內氧化壓力顯著提高，表示脂質過氧化傷害昇高，影響程度魚油大於運動，且此影響具有加成性。建議服用魚油與激烈運動同時，得適量適時補充抗氧化劑，以緩和機體的氧化傷害。

Exercise may induce higher oxygen free radicals. Fish oil belong to ω-3 PUFA series that can decrease TG and influence plasma blood lipids. Howerever, some evidence suggests it may increases the lipid peroxidation in the cell. Purpose: To investigates the effects of high-intensity exercise with fish oil supplementation on the antioxidant defense system. Methods: Thirty-three (33) healthy males (20.3±1.4 yrs; 64.3±7.9 kg) were randomly assigned into four groups, which were given a combinations of dietary fish oil (9 g /day), exercise (intensity 85~90%HRmax reserve), and placebos. The experiment lasted for 4 weeks. Venous samples were obtained prior to exercise and within 5 min after exercise. Four venous blood samples were also taken during the four-week period. Blood from all groups was analyzed for SOD, GSH-Px, t-GSH, TAA, MDA. A Generalized Estimating Equations (G.E.E.) method was used for data analysis. Results: The fish oil groups SOD activity significantly increased 11.27 kU/g-HB (p<.05) and decrease in GSH-Px levels, but not appear in the exercise groups. Following exercise in the fish oil group, t-GSH increased significantly 16.07% (p<.05). MDA increased significantly after exercise in both exercise-alone and exercise/fish oil groups 0.12 and 0.27 mmole/ml (p<.05) respectively. Conclusions: exercise and fish oil can cause an increase in oxidative stress separately. Also, these two effects are additive.